Impact of Colonoscopy Bowel Preparation on Intestinal Microbiota

The gut microbiota is important in maintaining human health, but numerous factors have the potential to alter its composition. Our aim was to examine the impact of a standard bowel preparation on the intestinal microbiota using two different techniques. Fifteen subjects undergoing colonoscopy consumed a bowel preparation comprised of 10 mg bisacodyl and 2 L polyethylene glycol. The microbiota of stool samples, collected one month before, one week before (pre-colonoscopy), and one week, one month, and three to six months after colonoscopy (post-colonoscopy) was evaluated. Two samples were taken three to six months apart from five healthy subjects who did not undergo colonoscopy. Universal primers targeting the V2-V3 region of the 16S rRNA gene were used to PCR amplify all samples for denaturing gradient gel electrophoresis (PCR-DGGE). Pre- and post-colonoscopy samples were compared using Dice’s similarity coefficients. Three samples from ten subjects who underwent colonoscopy, and both samples from the five subjects who didn’t, were used for high-throughput sequencing of the V1-V3 region of the 16S rRNA gene. Samples were curated and analysed in Mothur. Results of the DGGE analyses show that the fecal microbiota of a small number of subjects had short-term changes. High-throughput sequencing results indicated that the variation between the samples of subjects who underwent colonoscopy was no greater than the variation observed between samples from subjects who did not. We conclude that bowel preparation does not have a lasting effect on the composition of the intestinal microbiota for the majority of subjects.
Type
Collection
Title
Impact of Colonoscopy Bowel Preparation on Intestinal Microbiota
Brief Title
Impact of Colonoscopy on Gut Microbiota
Alternate Title
Impact of Colonoscopy Bowel Preparation on Gut Microbiota
Collection Type
Repository
Access Privileges
Research School of Biology
DOI - Digital Object Identifier
10.4225/13/511C71F8612C3
Metadata Language
English
Data Language
English
Significance Statement
It shows that for the majority of subjects, colonoscopy preparation does not significantly alter the microbial community of the gut.
Brief Description
The gut microbiota of subjects was assessed before and after consumption of a colonoscopy preparation, to determine whether or not colonoscopy preparation perturbs the gut microbiota. We found that for the majority of subjects it did not.
Full Description
The gut microbiota is important in maintaining human health, but numerous factors have the potential to alter its composition. Our aim was to examine the impact of a standard bowel preparation on the intestinal microbiota using two different techniques. Fifteen subjects undergoing colonoscopy consumed a bowel preparation comprised of 10 mg bisacodyl and 2 L polyethylene glycol. The microbiota of stool samples, collected one month before, one week before (pre-colonoscopy), and one week, one month, and three to six months after colonoscopy (post-colonoscopy) was evaluated. Two samples were taken three to six months apart from five healthy subjects who did not undergo colonoscopy. Universal primers targeting the V2-V3 region of the 16S rRNA gene were used to PCR amplify all samples for denaturing gradient gel electrophoresis (PCR-DGGE). Pre- and post-colonoscopy samples were compared using Dice’s similarity coefficients. Three samples from ten subjects who underwent colonoscopy, and both samples from the five subjects who didn’t, were used for high-throughput sequencing of the V1-V3 region of the 16S rRNA gene. Samples were curated and analysed in Mothur. Results of the DGGE analyses show that the fecal microbiota of a small number of subjects had short-term changes. High-throughput sequencing results indicated that the variation between the samples of subjects who underwent colonoscopy was no greater than the variation observed between samples from subjects who did not. We conclude that bowel preparation does not have a lasting effect on the composition of the intestinal microbiota for the majority of subjects.
Contact Email
claire.obrien@anu.edu.au
Principal Investigator
Claire O'Brien
Supervisors
Professor Paul Pavli; Dr Gwen Allison
Collaborators
Dr Florian Grimpen
Fields of Research
1108 - Medical Microbiology; 110801 - Medical Bacteriology; 0605 - Microbiology
Socio-Economic Objective
920499 - Public Health (excl. Specific Population Health) not elsewhere classified; 9201 - Clinical Health (Organs, Diseases and Abnormal Conditions)
Type of Research Activity
Experimental development
Date Coverage
2013
2023
Time Period
20th century
Date of data creation
2011
Year of data publication
2013
Creator(s) for Citation
Claire
O’Brien
Gwen
Allison
Florian
Grimpen
Paul
Pavli
Publisher for Citation
The Australian National University Data Commons
Publications
Impact of colonoscopy bowel preparation on intestinal microbiota
Plos One (2013)
Access Rights
All files will be made accessible to the public upon publication in Plos One, 2013.
Retention Period
Until Dec 2023
Disposal Date
2024
Extent or Quantity
41 (40 sff files, 1 metadata file)
Data Management Plan
No
Status: Published
Published to:
  • Australian National University
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