Impact of Colonoscopy Bowel Preparation on Intestinal Microbiota

TitleImpact of Colonoscopy Bowel Preparation on Intestinal Microbiota
Brief TitleImpact of Colonoscopy on Gut Microbiota
Alternate TitleImpact of Colonoscopy Bowel Preparation on Gut Microbiota
Collection TypeRepository
Access PrivilegesResearch School of Biology
DOI - Digital Object Identifier10.4225/13/511C71F8612C3
Metadata LanguageEnglish
Data LanguageEnglish
Significance StatementIt shows that for the majority of subjects, colonoscopy preparation does not significantly alter the microbial community of the gut.
Brief DescriptionThe gut microbiota of subjects was assessed before and after consumption of a colonoscopy preparation, to determine whether or not colonoscopy preparation perturbs the gut microbiota. We found that for the majority of subjects it did not.
Full DescriptionThe gut microbiota is important in maintaining human health, but numerous factors have the potential to alter its composition. Our aim was to examine the impact of a standard bowel preparation on the intestinal microbiota using two different techniques.

Fifteen subjects undergoing colonoscopy consumed a bowel preparation comprised of 10 mg bisacodyl and 2 L polyethylene glycol. The microbiota of stool samples, collected one month before, one week before (pre-colonoscopy), and one week, one month, and three to six months after colonoscopy (post-colonoscopy) was evaluated. Two samples were taken three to six months apart from five healthy subjects who did not undergo colonoscopy. Universal primers targeting the V2-V3 region of the 16S rRNA gene were used to PCR amplify all samples for denaturing gradient gel electrophoresis (PCR-DGGE). Pre- and post-colonoscopy samples were compared using Dice’s similarity coefficients. Three samples from ten subjects who underwent colonoscopy, and both samples from the five subjects who didn’t, were used for high-throughput sequencing of the V1-V3 region of the 16S rRNA gene. Samples were curated and analysed in Mothur.

Results of the DGGE analyses show that the fecal microbiota of a small number of subjects had short-term changes. High-throughput sequencing results indicated that the variation between the samples of subjects who underwent colonoscopy was no greater than the variation observed between samples from subjects who did not.

We conclude that bowel preparation does not have a lasting effect on the composition of the intestinal microbiota for the majority of subjects.
Principal InvestigatorClaire O'Brien
SupervisorsProfessor Paul Pavli
Dr Gwen Allison
CollaboratorsDr Florian Grimpen
Fields of Research1108 - Medical Microbiology
110801 - Medical Bacteriology
0605 - Microbiology
Socio-Economic Objective920499 - Public Health (excl. Specific Population Health) not elsewhere classified
9201 - Clinical Health (Organs, Diseases and Abnormal Conditions)
Type of Research ActivityExperimental development
Date Coverage
Date FromDate To
Time Period20th century
Date of data creation2011
Year of data publication2013
Creator(s) for Citation
Given NameSurname
Publisher for CitationThe Australian National University Data Commons

Identifier Type

Identifier Value

Publication Title
Impact of colonoscopy bowel preparation on intestinal microbiota
Publication Reference
Plos One (2013)

Access RightsAll files will be made accessible to the public upon publication in Plos One, 2013.
Retention PeriodUntil Dec 2023
Disposal Date2024
Extent or Quantity41 (40 sff files, 1 metadata file)
Data Management PlanNo
Status: Published
Published To:
- Australian National University
Identifier: anudc:4896


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